P a g e | 13 2.2 Ash Content Determination To calculate the amount of ash left over after burning organic matter in order to calculate the mineral content of both organic and non-organic bananas. 2.2.1 Materials •Muffle furnace •Porcelain crucibles •Desiccator •Analytical balance 2.2.2 Procedure Weighing: To guarantee precision, clean porcelain crucibles were weighed with an analytical scale. Sample Preparation: Each crucible contained around 1 gram of banana powder. Heating: To fully oxidise the organic material, the crucibles were heated overnight at 550°C in a muffle furnace. Cooling: To ascertain the amount of ash present, the crucibles were reweighed following their cooling in a desiccator. Calculation: The following formula was used to determine the ash content: Ash Content (%) =Weight of Ash/ Initial Sample Weight ×100 By removing the banana samples' organic material and leaving only the mineral residue, this process offers insight into the concentration of minerals (AOAC, 2019).