P a g e | 29
3.4 DPPH Free Radical Scavenging Activity via Spectrophotometer
Both organic and non-organic banana samples were tested for antioxidant activity using the
DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Using a spectrophotometer, absorbance was
measured at various levels to evaluate each sample's capacity to scavenge free radicals
DPPH.
Table 10: Absorbance Values for DPPH Free Radical Scavenging Activity of Organic
and Non-Organic Banana Samples
Concentration (ml)Sample A (Organic)Sample B (Non-Organic)
1 ml0.0440.046
2 ml0.0370.034
3 ml0.0310.028
4 ml0.0210.021
Graph 3.4: DPPH Free Radical Scavenging Activity of Organic Banana (Sample A) at
Different Concentrations
DPPH Free Radical Scavenging Activity of Organic
Bananas
0.0460.05
Absorbance0.040.034
0.028
0.03
0.021
0.02
0.01
0
012345
Concentration (ml)
As the sample volume increases, the absorbance values show a reduction with increasing
concentration, suggesting an improved DPPH free radical scavenging activity. At 4 ml (0.020),
the absorbance was the lowest, indicating higher antioxidant action at this dosage.